Author information: Follicle-stimulating hormone FSH was purified, for the first time, from immature Japanese eel, Anguilla japonica, and its biochemical properties were investigated. FSH was extracted from immature eel pituitaries and purified by gel-filtration on Sephadex Sex, and two step anion-exchange chromatography: Purification was performed using its molecular mass and the positive reaction with zimbabwe pornography stars eel je FSHbeta antiserum.
Ecological aspects of the Japanese eel, Anguilla japonica, collected from coastal areas of Japan.
The molecular mass of purified eel FSH was estimated to be approximately 33 kDa. Deglycosylation of these subunits led to a decrease in their molecular mass.
Cells reacting with anti-jeFSHbeta antiserum were observed in the proximal pars distalis of an immature eel pituitary, while jeLHbeta-immunoreactive cells were not detected. Gonadotropic activities of eel FSH were demonstrated in vitro by stimulating testosterone and ketotestosterone secretions in immature eel testes. Purified eel FSH stimulated the secretion of both androgens from the immature eel testis in a dose-dependent manner, similar to immature eel pituitary homogenate and recombinant eel FSH produced by yeast.
These results show that endogenous and recombinant FSH in this species possess similar activities, presumably stimulating the gametogenesis japan the sex steroid secretion during the early stages of gonadal development.